江汉大学学报（自然科学版）

本研究以4株对豇豆菌核病、豇豆枯萎病和豇豆煤霉病等病害具有拮抗作用的生防菌株为研究对象，组成一种复配生防菌剂。将不同菌株分别置于37℃和50℃培养箱中黑暗培养24 h以评估菌株耐热性。以大棚栽培的‘鄂豇豆14’品种作为试验材料，设置3个处理组评估复配菌剂CMF01对豇豆生长和产量的影响。结果表明，菌株J-5、M-4、LD-2在50℃下仍能生长，说明它们具有较强的耐高温能力；复配菌剂处理显著提高豇豆生长初期的生长速率，使豇豆提前到达开花期；与对照组和多菌灵处理组相比，复配菌剂处理可提高豇豆光合系统叶绿素含量，维持SOD、POD、CAT等抗氧化酶活性，减轻膜脂过氧化伤害，促使豇豆豆荚和根系鲜重显著增高，最终起到增产作用。

This study focused on four biocontrol strains that have antagonistic effects against diseases such as Sclerotinia sclerotiorum, Fusarium oxysporum, and Pseudocercospora cruenta in cowpeas,and developed a mixed biocontrol agent. The different strains were placed in an incubator at 37 ℃ and 50 ℃,respectively,and cultured in the dark for 24 hours to assess their heat resistance. The cowpea variety ' E-Jiangdou 14' cultivated in a greenhouse was used as the experimental material. Three treatment groups were set up to evaluate the effects of the mixed biocontrol agent CMF01 on cowpea growth and yield. The results showed that strains J-5,M-4,and LD-2 could still grow at 50 ℃,indicating their resistance to high temperature. The mixed biocontrol agent treatment significantly increased the growth rate of cowpea during the vine-drawing stage and promoted earlier flowering. Compared with the control group and the carbendazim treatment group,the mixed biocontrol agent treatment also increased the chlorophyll content in cowpea leaves,maintained the activities of antioxidant enzymes such as SOD,POD,and CAT,reduced the damage of membrane lipid peroxidation,significantly promoted the fresh weight of pods and roots,and finally increased the yield.